Retinoic Acid Receptor alpha antibody (pSer77)

Catalog No. ABIN7223940

The Rabbit Polyclonal anti-Retinoic Acid Receptor alpha antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect Retinoic Acid Receptor alpha in samples from Human, Mouse and Rat.

Quick Overview for Retinoic Acid Receptor alpha antibody (pSer77) (ABIN7223940)

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoic Acid Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Retinoic Acid Receptor alpha Antibody

Binding Specificity: pSer77

Purpose: RARα (phospho Ser77) Polyclonal Antibody

Specificity: Phospho-RARα (S77) Polyclonal Antibody detects endogenous levels of RARα protein only when phosphorylated at S77.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human RARalpha Phospho-Ser77

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Retinoic Acid Receptor alpha

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Alternative Name: RARalpha

Background: Rabbit Anti-RARα (phospho Ser77) Polyclonal Antibody,RARA, NR1B1, Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1,RARA (retinoic acid receptor alpha)represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. RARA has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.,Retinoic acid receptor alpha

Molecular Weight: observerd band 45kDa

Gene ID: 5914

UniProt: P10276

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, S100 Proteins