STK39 antibody (pSer311)

Catalog No. ABIN7221138

The Rabbit Polyclonal anti-STK39 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect STK39 in samples from Human, Mouse and Rat.

Quick Overview for STK39 antibody (pSer311) (ABIN7221138)

Target: STK39 (serine threonine Kinase 39 (STK39))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STK39 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STK39 Antibody

Binding Specificity: pSer311

Purpose: SPAK (phospho Ser311) Polyclonal Antibody

Specificity: Phospho-SPAK (S311) Polyclonal Antibody detects endogenous levels of SPAK protein only when phosphorylated at S311.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human SPAK Phospho-Ser311

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for STK39

Target: STK39 (serine threonine Kinase 39 (STK39))

Alternative Name: SPAK

Background: Rabbit Anti-SPAK (phospho Ser311) Polyclonal Antibody,STK39, SPAK, STE20/SPS1-related proline-alanine-rich protein kinase, Ste-20-related kinase, DCHT, Serine/threonine-protein kinase 39,STK39 encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.,STE20

Molecular Weight: observerd band 60kDa

Gene ID: 27347

UniProt: Q9UEW8