Splicing Factor 1 antibody (pSer82)

Catalog No. ABIN7224982

The Rabbit Polyclonal anti-Splicing Factor 1 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect Splicing Factor 1 in samples from Human, Mouse and Monkey.

Quick Overview for Splicing Factor 1 antibody (pSer82) (ABIN7224982)

Target: Splicing Factor 1 (SF1)

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Splicing Factor 1 Antibody

Binding Specificity: pSer82

Purpose: Splicing factor 1 (phospho Ser82) Polyclonal Antibody

Specificity: Phospho-Splicing factor 1 (S82) Polyclonal Antibody detects endogenous levels of Splicing factor 1 protein only when phosphorylated at S82.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Splicing factor 1 Phospho-Ser82

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: Splicing factor 1

Background: Rabbit Anti-Splicing factor 1 (phospho Ser82) Polyclonal Antibody,SF1, ZFM1, ZNF162, Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162,SF1 (splicing factor 1) encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants.,Splicing factor 1

Molecular Weight: observerd band 68kDa

Gene ID: 7536

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location