SHB antibody (pTyr246)

Catalog No. ABIN7213118

This anti-SHB antibody is a Rabbit Polyclonal antibody detecting SHB in WB and ELISA. Suitable for Human and Mouse.

Quick Overview for SHB antibody (pTyr246) (ABIN7213118)

Target: SHB (Src Homology 2 Domain Containing Adaptor Protein B (SHB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SHB Antibody

Binding Specificity: pTyr246

Purpose: Shb (phospho Tyr246) Polyclonal Antibody

Specificity: Phospho-Shb (Y246) Polyclonal Antibody detects endogenous levels of Shb protein only when phosphorylated at Y246.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Shb Phospho-Tyr246

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SHB

Target: SHB (Src Homology 2 Domain Containing Adaptor Protein B (SHB))

Alternative Name: Shb

Background: Rabbit Anti-Shb (phospho Tyr246) Polyclonal Antibody,SHB, SH2 domain-containing adapter protein B,SHB (SH2 homology protein in B/ beta -cells) is a 55-59 kDa cytoplasmic adaptor protein that serves as a link between phosphotyrosine residues and downstream signaling pathways. SHB is ubiquitously expressed, and binds tyrosine kinase receptors such as FGFR1, VEGFR2, and the TCR (zeta -chain) following their activation. It is highly modular, and through a variety of motifs, is able to bind multiple, structurally unrelated proteins that collectively generate a signal transduction network. Human SHB is 509 amino acids (aa) in length. It contains an N-terminal Pro-rich region that binds SH3 domain-containing proteins (aa 19-45), a central PTB domain that binds select aa motifs, and a C-terminal SH2 domain that interacts with phosphotyrosines (aa 410-504). There are at least nine utilized phosphorylation sites. There are two isoform variants. One is 66-68 kDa in size, and possesses an 87 aa Pro-rich N-terminal extension. This MW may increase to 77-80 kDa following posttranslational processing. A second variant shows an 18 aa substitution for aa 280-509. Over aa 2-128, human SHB shares 91 % aa identity with mouse SHB.,SH2 domain-containing adapter protein B

Molecular Weight: observerd band 55kDa

Gene ID: 6461

UniProt: Q15464