SMC1A antibody (pSer957)

Catalog No. ABIN7225226

This anti-SMC1A antibody is a Rabbit Polyclonal antibody detecting SMC1A in WB, ELISA and IHC (p). Suitable for Human and Mouse.

Quick Overview for SMC1A antibody (pSer957) (ABIN7225226)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMC1A Antibody

Binding Specificity: pSer957

Purpose: SMC1 (phospho Ser957) Polyclonal Antibody

Specificity: Phospho-SMC1 (S957) Polyclonal Antibody detects endogenous levels of SMC1 protein only when phosphorylated at S957.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human SMC1 Phospho-Ser957

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1

Background: Rabbit Anti-SMC1 (phospho Ser957) Polyclonal Antibody,SMC1A, DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8,Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. SMC1A (structural maintenance of chromosomes 1A), which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in SMC1A result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.,Structural maintenance of chromosomes protein 1A

Molecular Weight: observerd band 143kDa

Gene ID: 8243

UniProt: Q14683

Pathways: Stem Cell Maintenance