TAF II p135/p105 (AA 910-990) antibody

Catalog No. ABIN7224590

The Rabbit Polyclonal anti-not_set antibody has been validated for ELISA, WB and IHC (p). It is suitable to detect not_set in samples from Human and Mouse.

Quick Overview for TAF II p135/p105 (AA 910-990) antibody (ABIN7224590)

Target: TAF II p135/p105

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 910-990

Purpose: TAF II p135/p105 Polyclonal Antibody

Specificity: TAF II p135/p105 Polyclonal Antibody detects endogenous levels of TAF II p135/p105 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human TAF II p135/p105 at AA range: 910-990

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: TAF II p135/p105

Background: Rabbit Anti-TAF II p135/p105 Polyclonal Antibody,TAF4, TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135, Transcription initiation factor TFIID subunit 4, RNA polymerase II TBP-associated factor subunit C, TBP-associated factor 4, Transcription initiation factor TFIID 130 kDa subunit, TAF(II)130,Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF4 (TATA-box binding protein associated factor 4) encodes one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid, thyroid hormone and vitamin D3 receptors. In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases.,Transcription initiation factor TFIID subunit 4

Molecular Weight: observerd band 110kDa

Gene ID: 6874, 6875