TCF4/12 (AA 550-630) antibody

Catalog No. ABIN7217260

The Rabbit Polyclonal anti-not_set antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect not_set in samples from Human, Mouse and Rat.

Quick Overview for TCF4/12 (AA 550-630) antibody (ABIN7217260)

Target: TCF4/12

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 550-630

Purpose: TCF-4/12 Polyclonal Antibody

Specificity: TCF-4/12 Polyclonal Antibody detects endogenous levels of TCF-4/12 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human TCF-4/12 at AA range: 550-630

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: TCF4/12

Alternative Name: TCF-4/12

Background: Rabbit Anti-TCF-4/12 Polyclonal Antibody,TCF4, BHLHB19, ITF2, SEF2, Transcription factor 4, TCF-4, Class B basic helix-loop-helix protein 19, bHLHb19, Immunoglobulin transcription factor 2, ITF-2, SL3-3 enhancer factor 2, SEF-2, TCF12, BHLHB20, HEB, HTF4, Transcription factor 12,TCF4 encodes transcription factor 4, a basic helix-loop-helix transcription factor. The Transcription factor 4 recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described.,Transcription factor 4

Molecular Weight: observerd band 60kDa

Gene ID: 6925, 6938