Topoisomerase I antibody (Internal Region)

Catalog No. ABIN7224740

This anti-Topoisomerase I antibody is a Rabbit Polyclonal antibody detecting Topoisomerase I in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for Topoisomerase I antibody (Internal Region) (ABIN7224740)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Topoisomerase I Antibody

Binding Specificity: Internal Region

Purpose: Topo I Polyclonal Antibody

Specificity: Topo I Polyclonal Antibody detects endogenous levels of Topo I protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Topo I

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: Topo I

Background: Rabbit Anti-Topo I Polyclonal Antibody,TOP1, DNA topoisomerase 1, DNA topoisomerase I,TOP1 (topoisomerase (DNA) I) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. TOP1 is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.,DNA topoisomerase 1

Molecular Weight: observerd band 90kDa

Gene ID: 7150

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance