NFE4 antibody (acLys43)

Catalog No. ABIN7212636

This Rabbit Polyclonal antibody specifically detects NFE4 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for NFE4 antibody (acLys43) (ABIN7212636)

Target: NFE4 (Transcription Factor NF-E4 (NFE4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFE4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NFE4 Antibody

Binding Specificity: acLys43

Purpose: NF-E4 (Acetyl Lys43) Polyclonal Antibody

Specificity: Acetyl-NF-E4 (K43) Polyclonal AntibodySynthesized peptide derived from the human NF-E4 around the acetylation site of K43.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the human NF-E4 around the acetylation site of K43

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NFE4

Target: NFE4 (Transcription Factor NF-E4 (NFE4))

Alternative Name: NF-E4

Background: Rabbit Anti-NF-E4 (Acetyl Lys43) Polyclonal Antibody,NFE4, Transcription factor NF-E4,The erythroid-specific protein encoded by NFE4, and the ubiquitous transcription factor CP2, form the stage selector protein(SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG(CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform(22 kDa) acts as an activator, the short isoform(14 kDa) has been shown to repress gamma-globin gene expression. NFE4 is located in an intron of the FBXL13 gene on the opposite strand.,Transcription factor NF-E4

Molecular Weight: observerd band 19kDa

Gene ID: 58160

UniProt: Q86UQ8