TFF1 antibody

Catalog No. ABIN7212348

This anti-TFF1 antibody is a Mouse Monoclonal antibody detecting TFF1 in WB, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for TFF1 antibody (ABIN7212348)

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5E3

Product Details anti-TFF1 Antibody

Purpose: HP-1α Mouse Monoclonal Antibody (5E3)

Specificity: The antibody detects endogenous HP-1α protein.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Immunogen: Recombinant Protein of HP-1alpha

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:50-1:2000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: HP-1alpha

Background: Mouse Anti-HP-1α Mouse Monoclonal Antibody (5E3),Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha,CBX5 (chromobox 5) encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. CBX5 has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.,Chromobox protein homolog 5 (Antigen p25) (Heterochromatin protein 1 homolog alpha) (HP1 alpha)

Molecular Weight: observerd band 22kDa

Gene ID: 23468

UniProt: P45973

Pathways: EGFR Signaling Pathway