TRIM25 antibody (AA 180-260)

Catalog No. ABIN7225004

The Rabbit Polyclonal anti-TRIM25 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect TRIM25 in samples from Human.

Quick Overview for TRIM25 antibody (AA 180-260) (ABIN7225004)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRIM25 Antibody

Binding Specificity: AA 180-260

Purpose: EFP Polyclonal Antibody

Specificity: EFP Polyclonal Antibody detects endogenous levels of EFP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human EFP at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: EFP

Background: Rabbit Anti-EFP Polyclonal Antibody,TRIM25, EFP, RNF147, ZNF147, E3 ubiquitin/ISG15 ligase TRIM25, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147,The protein encoded by TRIM25 (tripartite motif containing 25) is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of TRIM25 is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.,E3 ubiquitin

Molecular Weight: observerd band 71kDa

Gene ID: 7706

UniProt: Q14258