UBD antibody (Internal Region)

Catalog No. ABIN7226618

This anti-UBD antibody is a Rabbit Polyclonal antibody detecting UBD in WB and ELISA. Suitable for Human.

Quick Overview for UBD antibody (Internal Region) (ABIN7226618)

Target: UBD (Ubiquitin D (UBD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-UBD Antibody

Binding Specificity: Internal Region

Purpose: FAT10 Polyclonal Antibody

Specificity: FAT10 Polyclonal Antibody detects endogenous levels of FAT10 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human FAT10

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for UBD

Target: UBD (Ubiquitin D (UBD))

Alternative Name: FAT10

Background: Rabbit Anti-FAT10 Polyclonal Antibody,UBD, FAT10, Ubiquitin D, Diubiquitin, Ubiquitin-like protein FAT10,FAT10, also designated Ubiquitin D or Diubiquitin, is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin. Each domain contains two cysteines, along with a free C-terminal diglycine motif required for FAT10 conjugate formation. FAT10 is inducible by interferon-g and tumor necrosis factor a (TNF). The FAT10 protein interacts with MAD2, a component of the spindle checkpoint, and plays a role in antigen presentation, cytokine response, apoptosis and mitosis. It may also regulate cell growth during dendritic cell or B cell activation and development. FAT10 mRNA is expressed mainly in some dendritic cells and lymphoblastoid lines and in other specific cells subsequent to interferon-g induction. The human FAT10 gene, designated UBD, maps to chromosome 6p21. and is overexpressed in the tumors of various epithelial cancers.,Ubiquitin D

Molecular Weight: observerd band 18kDa

UniProt: O15205

Pathways: Ubiquitin Proteasome Pathway