V-ATPase D1 antibody (Internal Region)

Catalog No. ABIN7217604

This anti-V-ATPase D1 antibody is a Rabbit Polyclonal antibody detecting V-ATPase D1 in ELISA and WB. Suitable for Human, Mouse and Rat.

Quick Overview for V-ATPase D1 antibody (Internal Region) (ABIN7217604)

Target: V-ATPase D1 (VHA36-1) (Vacuolar H[+] ATPase Subunit 36-1 (VHA36-1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This V-ATPase D1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-V-ATPase D1 Antibody

Binding Specificity: Internal Region

Purpose: V-ATPase D1 Polyclonal Antibody

Specificity: V-ATPase D1 Polyclonal Antibody detects endogenous levels of V-ATPase D1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human V-ATPase D1

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for V-ATPase D1

Target: V-ATPase D1 (VHA36-1) (Vacuolar H[+] ATPase Subunit 36-1 (VHA36-1))

Alternative Name: V-ATPase D1

Background: Rabbit Anti-V-ATPase D1 Polyclonal Antibody,ATP6V0D1, ATP6D, VPATPD, V-type proton ATPase subunit d 1, V-ATPase subunit d 1, 32 kDa accessory protein, V-ATPase 40 kDa accessory protein, V-ATPase AC39 subunit, p39, Vacuolar proton pump subunit d 1,ATP6V0D1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously.,V-type proton ATPase subunit d 1

Molecular Weight: observerd band 40kDa

Gene ID: 9114

UniProt: P61421