Bassoon antibody (AA 786-1041)

Catalog No. ABIN7233033

This anti-Bassoon antibody is a Rabbit Polyclonal antibody detecting Bassoon in IHC, WB and IF. Suitable for Human.

Quick Overview for Bassoon antibody (AA 786-1041) (ABIN7233033)

Target: Bassoon (BSN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bassoon antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Bassoon Antibody

Binding Specificity: AA 786-1041

Purpose: Anti-Bassoon Rabbit Polyclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat Bassoon. Multiple isoforms are detected.

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Fusion protein corresponding to aa786-1041 (N-terminal) of human Bassoon (accession no. NM_003458.3).

Application Details

Application Notes: Immunoblotting: use at 1 μg/mL.
Immunohistochemistry: use at 1-10 μg/mL.
Immunofluorescence: use at 1-10 μg/mL.
Positive control: Rat brain tissue lysate
These are recommended concentrations,
Enduser should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for Bassoon

Target: Bassoon (BSN)

Alternative Name: BSN

Background: Protein bassoon,Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:12812759). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (PubMed:19380881). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5 (By similarity). Mediates also synapse to nucleus communication leading to rUniProtKB:O88778, PubMed:12812759, PubMed:19380881}.,Bassoon, a 420 kDa protein, is localized at presynaptic nerve terminals and appears to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The C-terminal regions of the polyglutamine domains are of interest since abnormal amplification of this region in some human proteins, such as Huntingtin, can cause late-onset neuro- degeneration.,Cytoplasm, Cell junction, synapse, presynaptic active zone, Cytoplasm, cytoskeleton, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane,Zinc finger protein 231

UniProt: Q9UPA5