GABARAP antibody (C-Term)

Catalog No. ABIN7233059

The Rabbit Polyclonal anti-GABARAP antibody has been validated for WB and IF/ICC. It is suitable to detect GABARAP in samples from Human.

Quick Overview for GABARAP antibody (C-Term) (ABIN7233059)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABARAP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Product Details anti-GABARAP Antibody

Binding Specificity: C-Term

Purpose: Anti-GABARAP Rabbit Polyclonal Antibody

Specificity: This antibody recognizes human and mouse GABARAP.

Cross-Reactivity: Human, Mouse

Immunogen: Synthetic peptide corresponding to the C-terminus of human GABARAP

Application Details

Application Notes: Immunoblotting: use at dilution of 1:1,000. A band of ~14-16 kDa is detected. Other bands observed at ~40 kDa (complex) and ~26 kDa (dimer).
Immunofluorescence: use at dilution of 1:100
These are recommended working dilutions.
Endusers should determine optimal dilutions for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Alternative Name: GABARAP

Background: γ-aminobutyric acid receptor-associated protein,Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355). Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538). Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205). Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205). Involved in apoptosis (PubMed:15977068). {PubMed:15169837, PubMed:15977068, PubMed:20562859, PubMed:22948227, PubMed:25684205, PubMed:31006538, PubMed:9892355}.,Gamma-aminobutyric acid A receptors (GABA(A) receptors) are ligand-gated chloride channels that mediate inhibitory neurotransmission. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. In addition, GABARAP has an important role in autophagy, it is crucial for autophagosome formation and sequestering cytosolic cargo into double-membrane vesicles which is then followed by degradation after fusion with lysosomes.,Cytoplasmic vesicle, autophagosome membrane, Endomembrane system, Cytoplasm, cytoskeleton, Golgi apparatus membrane, Cytoplasmic vesicle,GABA(A receptor-associated protein, MM46

UniProt: O95166

Pathways: Autophagy