PVALB antibody

Catalog No. ABIN7233200

The Mouse Monoclonal anti-PVALB antibody has been validated for WB, ELISA and ICC. It is suitable to detect PVALB in samples from Measles Virus.

Quick Overview for PVALB antibody (ABIN7233200)

Target: PVALB (Parvalbumin (PVALB))

Reactivity: Measles Virus

Host: Mouse

Clonality: Monoclonal

Conjugate: This PVALB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: 9H4

Product Details anti-PVALB Antibody

Purpose: Anti-Measles Phosphoprotein Mouse Monoclonal Antibody

Specificity: This antibody reacts with Measles virus phosphoprotein.

Cross-Reactivity: Measles Virus

Immunogen: Recombinant Measles virus phosphoprotein.

Isotype: IgG1

Application Details

Application Notes: ELISA: use at 0.1-1.0 μg/mL
Immunoblotting:
User should use at 1-5 μg/mL. A band of ~70 kDa is detected
Immunocytochemistry: use at 2- 10 μg/mL on virus-infected cells.
These are recommended concentrations.
End user should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 μg/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.

Buffer: 0.1M Tris, 0.1M glycine, 2 % sucrose

Storage: -20 °C,-80 °C

Storage Comment: This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

Target Details for PVALB

Target: PVALB (Parvalbumin (PVALB))

Alternative Name: P/V

Background: Phosphoprotein,Essential component of the RNA polymerase and the nascent chain assembly complex. Also required during RNA synthesis.,Measles virus (MeV) is an enveloped RNA virus in the Morbillivirus genus of the Paramyxoviridae family. Its non- segmented, negative-sense, single- stranded RNA genome is encapsidated by the viral nucleoprotein (N) within a helical nucleocapsid. Transcription and replication are carried out on this N:RNA complex by the viral polymerase complex which consists of two components, the large protein (L) and the phosphoprotein (P). MeV N consists of two regions: a structured N-terminal moiety, NCORE (aa 1â€""400), which contains all the regions necessary for self-assembly and RNA-binding, and a C-terminal domain, NTAIL(aa 401â€""525) that is intrinsically unstructured and is exposed at the surface of the viral nucleocapsid.,Protein P

UniProt: P35974