GPR18 antibody (Extracellular)

Catalog No. ABIN7237662

This Rabbit Polyclonal antibody specifically detects GPR18 in WB, IHC, FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for GPR18 antibody (Extracellular) (ABIN7237662)

Target: GPR18 (G Protein-Coupled Receptor 18 (GPR18))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR18 Antibody

Binding Specificity: AA 7-22, Extracellular

Purpose: A Rabbit Polyclonal antibody to GPR18 (extracellular)

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - 13 out of 16 amino acid residues identical Not recommended for human samples

Characteristics: Extracellular, N-terminus

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HNQLDLSNGSHPEEYK, corresponding to amino acid residues 7 - 22 of mouse GPR18

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235388)

Blocking Peptide: (ABIN7235388)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR18

Target: GPR18 (G Protein-Coupled Receptor 18 (GPR18))

Alternative Name: GPR18 

Background: G-Protein Coupled Receptor 18, N-Arachidonyl Glycine Receptor, NAGly Receptor, GPCRW,G protein coupled receptors (GPCRs) comprise a large superfamily of receptors characterized by a seven transmembrane domain structure and an ability to activate intracellular transducer G proteins. Over 800 GPCRs in five main families have been identified in eukaryotes on the basis of genomic sequence analysis 1-2. These receptors can be activated by hormones, neurotransmitters, odorants, light, or pheromones. GPCRs mediate the signaling of extracellular stimuli to intracellular responses via activation of heterotrimeric G proteins and their subsequent interaction with effector proteins. GPCRs have been implicated in a number of physiological functions including development of anxiety, behavior, homeostasis, cognition, appetite, and drug addiction 3. The GPCR family contains a diverse group of cell-surface mediators of signal transduction, its size exceeding other cell-surface protein receptors including the tyrosine kinase receptors, guanylyl cyclase receptors and ligand-gated ion channels 4. The murine homolog of GPR18 was first isolated in 1996 5. A potential agonist for this orphan receptor was proposed only in 2006 when N-arachidonylglycine (NAGly) was shown to increase calcium concentration and inhibit forskolin induced cAMP production in CHO cells in a pertussis sensitive manner 6. GPR18 shares low sequence homology with the cannabinoid receptors CB1R and CB2R (~13 % and 8 %) and moderate identity with the putative cannabinoid receptor GPR55 (21 %). Nonetheless, it has been related to the endocannabinoid system since a range of endogenous, phytogenic and synthetic cannabinoids have shown to modulate GPR18 7. GPR18 was shown to be constitutively active in melanomas, acting as an apoptosis inhibitor 8, while playing a reverse role in macrophage apoptosis, that is, signaling for cell death9. Both of these roles were mediated via Gαi pathways. Work on alternate cannabinoid compounds as potential agonist showed differential activation of Gαq / Gαi signaling suggesting biased agonism in pathway selection10.

Alternative names: G-Protein Coupled Receptor 18, N-Arachidonyl Glycine Receptor, NAGly Receptor, GPCRW

Gene ID: 110168

NCBI Accession: NM_001098200

UniProt: Q8K1Z6