GPR50 antibody (Intracellular)

Catalog No. ABIN7237666

The Rabbit Polyclonal anti-GPR50 antibody has been validated for WB. It is suitable to detect GPR50 in samples from Mouse.

Quick Overview for GPR50 antibody (Intracellular) (ABIN7237666)

Target: GPR50 (G Protein-Coupled Receptor 50 (GPR50))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR50 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GPR50 Antibody

Binding Specificity: AA 307-321, Intracellular

Purpose: A Rabbit Polyclonal antibody to GPR50

Specificity: Intracellular, C-terminus.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - identical,Human - 14 out of 15 amino acid residues identical

Characteristics: Intracellular, C-terminus

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ESFRREYWTIFHAMR, corresponding to amino acid residues 307 - 321 of mouse GPR50

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235419)

Blocking Peptide: (ABIN7235419)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR50

Target: GPR50 (G Protein-Coupled Receptor 50 (GPR50))

Alternative Name: GPR50 

Background: G Protein-Coupled Receptor 50, Melatonin-Related Receptor,G protein-coupled receptor 50 (GPR50), previously known as the melatonin-related receptor, share 45 % sequence identity to melatonin receptors MT1 and MT2 but it does not bind melatonin or any other known ligand and therefore remains an orphan receptor without a clear function1,2.GPR50 belongs to the G protein coupled receptors (GPCRs) family, a large group of receptors characterized by seven trans membrane helices that transmit a signal from the outside cellular surface into the inside of the cell through conformational changes. GPCRs mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants, and so have great potential as therapeutic targets for a broad spectrum of diseases1.GPR50 was identified as a risk gene for bipolar disorder and major depression and it was shown to have a role in neural development and sterol metabolism, it was also shown to be related to mood disorders, adaptive thermogenesis and torpor response (the body's response to starvation)2. In addition, it was shown to have a dramatic effect on neurite outgrowth, supporting a likely role in neural development and it was also found to be expressed in various types of cancer3.GPR50 is expressed in the pituitary, hypothalamus, cortex, midbrain, pons, amygdala, and in several brainstem nuclei3,4. Due to its involvement in a wide range of mental conditions and disease, GPR50 is a major target for research and drug development.

Alternative names: G Protein-Coupled Receptor 50, Melatonin-Related Receptor

Gene ID: 14765

NCBI Accession: NM_004224

UniProt: O88495