HOMER1 antibody (Intracellular)

Catalog No. ABIN7237693

The Guinea Pig Polyclonal anti-HOMER1 antibody has been validated for WB and IHC. It is suitable to detect HOMER1 in samples from Rat.

Quick Overview for HOMER1 antibody (Intracellular) (ABIN7237693)

Target: HOMER1 (Homer Homolog 1 (HOMER1))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This HOMER1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HOMER1 Antibody

Binding Specificity: AA 293-296, Intracellular

Purpose: A Guinea Pig Polyclonal Antibody to Homer1

Specificity: Intracellular

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): The antibody will not recognize Homer1 short isoform 3 (also known as Homer1a), but will recognize isoform 2 (also known as Homer1b).

Predicted Reactivity: Mouse,human - identical

Characteristics: Intracellular

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KQEIDNARELQEQR, corresponding to amino acid residues 293-296 of rat Homer1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235762)

Blocking Peptide: (ABIN7235762)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HOMER1

Target: HOMER1 (Homer Homolog 1 (HOMER1))

Alternative Name: HOMER1

Background: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47,Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1.The long form of Homer1 assembles group 1 metabotropic glutamate receptors mGluR1 and mGluR5 in large macromolecular complexes at synapses as well as with components of the NMDA receptor complex at the neuronal cell membrane. In contrast, the short form of Homer1 lacks the ability of linking mGluR1/5 to synaptic proteins and functions as an endogenous negative modulator of the mGluR1/5-inositol 1,4,5-trisphosphate receptor signaling complex. Transgenic mice with overexpressed Homer1 in striatal medium spiny neurons demonstrate normal development of striatal structure and afferent/efferent connectivity. However, motor performance in behavioral tasks and striatal responses to the psychomotor stimulant amphetamine are significantly altered in these mice2.At concentrations

Alternative names: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47

Gene ID: 29546

NCBI Accession: NM_004272

UniProt: Q9Z214

Pathways: Skeletal Muscle Fiber Development