LRRC4 antibody (Extracellular)

Catalog No. ABIN7237706

The Rabbit Polyclonal anti-LRRC4 antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect LRRC4 in samples from Mouse.

Quick Overview for LRRC4 antibody (Extracellular) (ABIN7237706)

Target: LRRC4 (Leucine Rich Repeat Containing 4 (LRRC4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRRC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-LRRC4 Antibody

Binding Specificity: AA 507-520, Extracellular

Purpose: A Rabbit Polyclonal antibody to NGL-2/LRRC4 (extracellular)

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical,Human 13 out of 14 amino acid residues identical

Characteristics: Extracellular, N-terminus

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)STDTTDKMQTSLDE, corresponding to amino acid residues 507 - 520 of mouse LRRC4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235857)

Blocking Peptide: (ABIN7235857)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LRRC4

Target: LRRC4 (Leucine Rich Repeat Containing 4 (LRRC4))

Alternative Name: LRRC4 

Background: Netrin-G2 Ligand, Brain Tumor-Associated Protein BAG, Leucine-Rich Repeat-Containing Protein 4,The leucine-rich repeat (LRR) superfamily is a diverse protein family that consist of leucine-rich domains (LRR domains). The LRR domain is known to regulate protein-protein interaction or cell adhesion. Large portion of LRR proteins mediate the differentiation and development of nervous tissues1. One member of the LRR superfamily is leucine rich repeat containing protein 4 (LRRC4), also known as netrin-G ligand-2 (NGL-2), and serves as a receptor for netrin-G2 (NTNG2). In addition to the LRR domain LRRC4 consist of immunoglobulin (Ig) domain in the extracellular region, a transmembrane domain, and a cytoplasmic region that terminates in a protein-protein interaction domain (PDZ)-binding motif. LRRC4 regulates the formation of excitatory synapses and promotes axon differentiation2.Functionally, deletion of LRRC4 in mice was found to suppress excitatory synapse development and synaptic transmission. In addition, LRRC4 gene has been identified as tumor suppressor3. In human it has been implicated in autism spectrum disorder (ASD), schizophrenia, and intellectual disability. Moreover, netrin-G2, the presynaptic ligand of LRRC4, is linked to schizophrenia and bipolar disorder4-5.

Alternative names: Netrin-G2 Ligand, Brain Tumor-Associated Protein BAG, Leucine-Rich Repeat-Containing Protein 4

Gene ID: 192198

NCBI Accession: NM_022143

UniProt: Q99PH1

Pathways: Synaptic Membrane