NEO1 antibody (Extracellular)

Catalog No. ABIN7237724

This anti-NEO1 antibody is a Rabbit Polyclonal antibody detecting NEO1 in WB, IHC, FACS and LCI. Suitable for Mouse.

Quick Overview for NEO1 antibody (Extracellular) (ABIN7237724)

Target: NEO1 (Neogenin 1 (NEO1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-NEO1 Antibody

Binding Specificity: AA 701-715, Extracellular

Purpose: A Rabbit Polyclonal antibody to Neogenin (extracellular)

Specificity: Extracellular, N-term.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - identical

Characteristics: Extracellular, N-terminus

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KIRYRKASRKSDVTE, corresponding to amino acid residues 701 - 715 of mouse Neogenin

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235994)

Blocking Peptide: (ABIN7235994)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NEO1

Target: NEO1 (Neogenin 1 (NEO1))

Alternative Name: NEO1

Background: Neogenin 1, NEO1, NGN,Neogenin also known as NEO1 is a member of the immunoglobulin (Ig) superfamily. It has been reported to be involved in diverse physiology and pathology functions, including cell proliferation, differentiation and migration1. Neogenin was originally isolated from embryonic chicken cerebellum and reported to be homologous to the axon guidance receptor deleted in colorectal cancer (DCC)2.Neogenin consists of 4 N-terminal Ig-like domains, followed by 6 fibronectin type III-like domains, a single transmembrane helix, and an intracellular domain. Neogenin is capable to mediate attractive and repulsive axon guidance depending on the ligand identity. Two main ligands of neogenin are Netrin-1 (NET1) and repulsive guidance molecules (RGMs)3.Interaction of Neogenin with RGMs leads to cytoskeleton rearrangements via Rho GTPases, this results in growth cone collapse. In contrast interaction with Netrin-1 triggers attractive growth cone response3. Recently it was shown that Neogenin plays a role in the maintenance of iron homeostasis via interaction with HJV (members of the RGM family)4.

Alternative names: Neogenin 1, NEO1, NGN

Gene ID: 18007

NCBI Accession: NM_002499

UniProt: P97798

Pathways: Transition Metal Ion Homeostasis, Regulation of Muscle Cell Differentiation, Tube Formation