NEO1 antibody (Extracellular) (FITC)

Catalog No. ABIN7237728

This Rabbit Polyclonal antibody specifically detects NEO1 in FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for NEO1 antibody (Extracellular) (FITC) (ABIN7237728)

Target: NEO1 (Neogenin 1 (NEO1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEO1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-NEO1 Antibody (FITC)

Binding Specificity: AA 701-715, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Neogenin (extracellular) conjugated to the fluorescent dye FITC

Specificity: Extracellular, N-term.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - identical

Characteristics: Extracellular, N-terminus

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KIRYRKASRKSDVTE, corresponding to amino acid residues 701 - 715 of mouse Neogenin

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235994)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NEO1

Target: NEO1 (Neogenin 1 (NEO1))

Alternative Name: NEO1

Background: Neogenin 1, NEO1, NGN, NTN1R2,Neogenin also known as NEO1 is a member of the immunoglobulin (Ig) superfamily. It has been reported to be involved in diverse physiology and pathology functions, including cell proliferation, differentiation and migration1. Neogenin was originally isolated from embryonic chicken cerebellum and reported to be homologous to the axon guidance receptor deleted in colorectal cancer (DCC)2.Neogenin consists of 4 N-terminal Ig-like domains, followed by 6 fibronectin type III-like domains, a single transmembrane helix, and an intracellular domain. Neogenin is capable to mediate attractive and repulsive axon guidance depending on the ligand identity. Two main ligands of neogenin are Netrin-1 (NET1) and repulsive guidance molecules (RGMs)3.Interaction of Neogenin with RGMs leads to cytoskeleton rearrangements via Rho GTPases, this results in growth cone collapse. In contrast interaction with Netrin-1 triggers attractive growth cone response3. Recently it was shown that Neogenin plays a role in the maintenance of iron homeostasis via interaction with HJV (members of the RGM family)4.

Alternative names: Neogenin 1, NEO1, NGN, NTN1R2

Gene ID: 18007

NCBI Accession: NM_002499

UniProt: P97798

Pathways: Transition Metal Ion Homeostasis, Regulation of Muscle Cell Differentiation, Tube Formation