ARIH1 antibody (C-Term)

Catalog No. ABIN7265672

The Rabbit Polyclonal anti-ARIH1 antibody has been validated for WB. It is suitable to detect ARIH1 in samples from Human.

Quick Overview for ARIH1 antibody (C-Term) (ABIN7265672)

Target: ARIH1 (Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (ARIH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARIH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARIH1 Antibody

Binding Specificity: C-Term

Purpose: ARIH1 Rabbit pAb

Sequence: ARDAQERSRA ALQRYLFYCN RYMNHMQSLR FEHKLYAQVK QKMEEMQQHN MSWIEVQFLK KAVDVLCQCR ATLMYTYVFA FYLKKNNQSI IFENNQADLE NATEVLSGYL ERDISQDSLQ DIKQKVQDKY RYCESRRRVL LQHVHEGYEK DLWEYIED

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human ARIH1 (NP_005735.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for ARIH1

Target: ARIH1 (Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (ARIH1))

Alternative Name: ARIH1

Background: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets. The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes. Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear. According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report EIF4E2 ubiquitination leads to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2. In vitro, controls the degradation of the LINC (LInker of Nucleoskeleton and Cytoskeleton complex member SUN2 and may therefore have a role in the formation and localization of the LINC complex, and as a consequence, nuclear subcellular localization and nuclear morphology.,ARIH1,ARI,HARI,HHARI,UBCH7BP,Epigenetics & Nuclear Signaling,Cancer,Signal Transduction,Cell Biology & Developmental Biology,Ubiquitin,Endocrine & Metabolism,Amino acid metabolism,ARIH1

Gene ID: 25820

UniProt: Q9Y4X5