TNNT3 antibody (AA 147-256)

Catalog No. ABIN7267145

The Rabbit Polyclonal anti-TNNT3 antibody has been validated for WB. It is suitable to detect TNNT3 in samples from Human.

Quick Overview for TNNT3 antibody (AA 147-256) (ABIN7267145)

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNT3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TNNT3 Antibody

Binding Specificity: AA 147-256

Purpose: TNNT3 Rabbit pAb

Sequence: SMGANYSSYL AKADQKRGKK QTAREMKKKI LAERRKPLNI DHLGEDKLRD KAKELWETLH QLEIDKFEFG EKLKRQKYDI TTLRSRIDQA QKHSKKAGTP AKGKVGGRWK

Cross-Reactivity: Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 147-256 of human TNNT3 (NP_001036246.1).

Isotype: IgG

Application Details

Application Notes: WB,1:200 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for TNNT3

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Alternative Name: TNNT3

Background: The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein, also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).,TNNT3,TNTF,troponin T3,Signal Transduction,Cell Biology & Developmental Biology,Cytoskeleton,Microfilaments,TNNT3

Molecular Weight: 29kDa/30kDa/31kDa

Gene ID: 7140

UniProt: P45378