NSMCE1 antibody (AA 7-266)

Catalog No. ABIN7268951

The Rabbit Polyclonal anti-NSMCE1 antibody has been validated for WB. It is suitable to detect NSMCE1 in samples from Human.

Quick Overview for NSMCE1 antibody (AA 7-266) (ABIN7268951)

Target: NSMCE1 (Non-SMC Element 1 Homolog (NSMCE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSMCE1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NSMCE1 Antibody

Binding Specificity: AA 7-266

Purpose: NSMCE1 Rabbit pAb

Sequence: RMGVMTDVHR RFLQLLMTHG VLEEWDVKRL QTHCYKVHDR NATVDKLEDF INNINSVLES LYIEIKRGVT EDDGRPIYAL VNLATTSISK MATDFAENEL DLFRKALELI IDSETGFASS TNILNLVDQL KGKKMRKKEA EQVLQKFVQN KWLIEKEGEF TLHGRAILEM EQYIRETYPD AVKICNICHS LLIQGQSCET CGIRMHLPCV AKYFQSNAEP RCPHCNDYWP HEIPKVFDPE KERESGVLKS NKKSLRSRQH

Cross-Reactivity: Human, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 7-266 of human NSMCE1 (NP_659547.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for NSMCE1

Target: NSMCE1 (Non-SMC Element 1 Homolog (NSMCE1))

Alternative Name: NSMCE1

Background: RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. Within MAGE-RING ubiquitin ligase complex, MAGE stimulates and specifies ubiquitin ligase activity likely through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. Involved in maintenance of genome integrity, DNA damage response and DNA repair. NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair. MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors.,NSMCE1,NSE1,NSE1 homolog,Epigenetics & Nuclear Signaling,DNA Damage & Repair,NSMCE1

Molecular Weight: 30kDa

Gene ID: 197370

UniProt: Q8WV22

Pathways: Positive Regulation of Response to DNA Damage Stimulus