SARS-CoV-2 NSP13 antibody

Catalog No. ABIN7270148

The Rabbit Polyclonal anti-SARS-CoV-2 NSP13 antibody has been validated for WB and IF. It is suitable to detect SARS-CoV-2 NSP13 in samples from SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for SARS-CoV-2 NSP13 antibody (ABIN7270148)

Target: SARS-CoV-2 NSP13 (HEL) (SARS-CoV-2 Helicase (NSP13) (HEL))

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 NSP13 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-SARS-CoV-2 NSP13 Antibody

Purpose: SARS-CoV-2 NSP13 Rabbit pAb

Cross-Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein of SARS-CoV-2 NSP13.

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for SARS-CoV-2 NSP13

Target: SARS-CoV-2 NSP13 (HEL) (SARS-CoV-2 Helicase (NSP13) (HEL))

Alternative Name: NSP13

Target Type: Viral Protein

Background: [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.,NSP13

Gene ID: 43740578

UniProt: P0DTD1