Recombinant mNeonGreen antibody (Biotin)

Catalog No. ABIN7272891

The Alpaca Monoclonal anti-not_set antibody has been validated for ICC and IF. It is suitable to detect not_set in samples from Branchiostoma lanceolatum.

Quick Overview for Recombinant mNeonGreen antibody (Biotin) (ABIN7272891)

Target: mNeonGreen

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Branchiostoma lanceolatum

Host: Alpaca

Clonality: Monoclonal

Conjugate: Biotin

Application: Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 1E2

Product Details

Expression System: E.coli

Specificity: Recognizes mNeonGreen. Does not cross-react with any GFP- or dsRed, of TagBFP derivatives.

Purification: Produced in: E.coli

Immunogen: Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor

Application Details

Application Notes: Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute with 250 µL 50% glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.

Target Details

Target: mNeonGreen

Background: MNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.