Recombinant mTagBFP antibody (DBCO)

Catalog No. ABIN7272957

The Alpaca Monoclonal anti-not_set antibody has been validated for FACS, ICC and IF. It is suitable to detect not_set in samples from Entacmaea quadricolor.

Quick Overview for Recombinant mTagBFP antibody (DBCO) (ABIN7272957)

Target: mTagBFP

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Entacmaea quadricolor

Host: Alpaca

Clonality: Monoclonal

Conjugate: DBCO

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 1H7

Product Details

Expression System: E.coli

Specificity: Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2, mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657

Purification: Produced in: E.coli

Immunogen: Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2.

Application Details

Application Notes: Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute with 250 µL 50% glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.

Target Details

Target: mTagBFP

Background: TagRFP was engineered in 2007 from TurboRFP (derived from Entacmaea quadricolor). Tag RFP displayed an extended fluorescence lifetime and showed a robust photostability. In 2008 after further mutagenesis and the exchange of ~12 amino acid residues, this red-emitting protein turned into a blue-emitting protein TagBFP. Only several years later, in 2011 it a monomeric version of TagBFP was discribed: mTagBFP2.