TRIM25 antibody

Catalog No. ABIN7384377

The Rabbit Monoclonal anti-TRIM25 antibody has been validated for WB and IF. It is suitable to detect TRIM25 in samples from Human and Rat.

Quick Overview for TRIM25 antibody (ABIN7384377)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: R06-1H2

Product Details anti-TRIM25 Antibody

Purification: Affinity Purified

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:5000 IF 1:20-1:100

Restrictions: For Research Use only

Handling

Concentration: 300 μg/mL

Buffer: 50 mM Tris-Glycine( pH 7.4), 0.15M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: TRIM25

Background: E3 ubiquitin/ISG15 ligase TRIM25,EFP,Estrogen responsive finger protein,Estrogen-responsive finger protein,RING finger protein 147,RNF 147,RNF147,TRI25,TRI25,TRIM 25,Trim25,Tripartite motif containing 25,Tripartite motif containing protein 25,Tripartite motif protein TRIM25,Tripartite motif-containing protein 25,Tripartite motifcontaining25,Ubiquitin/ISG15-conjugating enzyme TRIM25,Z147,Zinc finger protein 147 (estrogen responsive finger protein),Zinc finger protein 147,Zinc finger protein-147,ZNF 147,ZNF147,The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Molecular Weight:

Observed_MW: 71kDa

Calculated_MW: 71kDa

Gene ID: 7706

UniProt: Q14258