beta Actin antibody

Catalog No. ABIN7384521

The Mouse Monoclonal anti-beta Actin antibody has been validated for WB and IF. It is suitable to detect beta Actin in samples from Human, Mouse and Zebrafish (Danio rerio).

Quick Overview for beta Actin antibody (ABIN7384521)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 2B2

Product Details anti-beta Actin Antibody

Purification: Protein A/G Purified

Immunogen: Recombinant human Beta actin protein

Isotype: IgG2a

Application Details

Application Notes: WB: 1:5000-10000, IP: 3 μg/sample, IF: 1:100-200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.1 % sodium azide, 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta actin

Background: ACTB, Actin beta, Actx, Actin cytoplasmic 1, b actin, A26C1A,Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR.

Molecular Weight:

Observed_MW: 43kDa

Calculated_MW: 43kDa

Gene ID: 57935

UniProt: Q7ZVF9

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction