CD8 alpha antibody (C-Term, Cytoplasmic Domain)

Catalog No. ABIN7449043

This Mouse Monoclonal antibody specifically detects CD8 alpha in FACS, IHC and IF (p). It exhibits reactivity toward Human.

Quick Overview for CD8 alpha antibody (C-Term, Cytoplasmic Domain) (ABIN7449043)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: C8-144B

Product Details anti-CD8 alpha Antibody

Binding Specificity: C-Term, Cytoplasmic Domain

Purpose: Mouse anti-CD8 alpha Monoclonal Antibody, Purified [C8/144B]

Purification: Purified

Immunogen: A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IHC: 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Restrictions: For Research Use only

Handling

Concentration: 250 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8 alpha

Background: Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain [taken from NCBI Entrez Gene (Gene ID: 925)].

Gene ID: 925

NCBI Accession: NP_001759

UniProt: P01732

Pathways: TCR Signaling