Recombinant TIM3 antibody (AA 22-201)

Catalog No. ABIN7449550

This Rabbit Monoclonal antibody specifically detects TIM3 in FACS, WB, IHC, ICC and IF (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant TIM3 antibody (AA 22-201) (ABIN7449550)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TIM3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: BLR033F

Product Details anti-TIM3 Antibody

Binding Specificity: AA 22-201

Purpose: Rabbit anti-TIM3 Recombinant Monoclonal Antibody [BLR033F]

Immunogen: residues 22-201 (ECD)

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: TIM3

Target Type: Virus

Background: Background: TIM3 (HAVCR2) is a cell surface receptor implicated in modulating innate and adaptive immune responses. It is generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. TIM3 regulates macrophage activation, inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses, and promotes immunological tolerance. In CD8+ cells TIM3 attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion.  [taken from the Universal Protein Resource (UniProt) Q8TDQ0].

Gene ID: 84868

NCBI Accession: NP_116171

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints