alpha Actinin 4 antibody (AA 1-50)

Catalog No. ABIN7449956

The Rabbit Polyclonal anti-alpha Actinin 4 antibody has been validated for WB and IP. It is suitable to detect alpha Actinin 4 in samples from Human and Mouse.

Quick Overview for alpha Actinin 4 antibody (AA 1-50) (ABIN7449956)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Actinin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-alpha Actinin 4 Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-alpha Actinin 4/ACTN4 Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine

Purification: Affinity Purified

Immunogen: Between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: alpha Actinin 4/ACTN4

Background: Background: Alpha Actinin 4 / ACTN4 is an F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). It is probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). It is involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. It links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). alpha Actinin 4 / ACTN4 may also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778) [taken from the Universal Protein Resource (UniProt) O43707].

Gene ID: 81

NCBI Accession: NP_004915

UniProt: O43707

Pathways: Proton Transport