ARHGEF12 antibody (AA 1494-1544)

Catalog No. ABIN7451849

The Rabbit Polyclonal anti-ARHGEF12 antibody has been validated for WB and IP. It is suitable to detect ARHGEF12 in samples from Human.

Quick Overview for ARHGEF12 antibody (AA 1494-1544) (ABIN7451849)

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF12 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ARHGEF12 Antibody

Binding Specificity: AA 1494-1544

Purpose: Rabbit anti-LARG Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1494 and 1544

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for ARHGEF12

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Alternative Name: LARG

Background: Background: LARG (leukemia-associated RhoGEF) is homologous to PDZ-RhoGEF and functions as a RhoA-specific guanine nucleotide exchange factor. RhoGEFs catalyze the exchange of GDP for GTP on the GTPase, Rho, to activate downstream signaling pathways. LARG contains a PDZ [post synaptic density protein (PSD95), Drosophila discs-large tumor suppressor (DIgA), and zonula occludens-1 protein (zo-1)] domain which is an 80-90 amino acid structural domain found in signaling proteins in bacteria, yeast, plants, and animals. LARG is proposed to play a role in cell polarity and microtubule dynamics and may act as a GTPase-activating protein (GAP) for the heterotrimeric Galpha(12) and Galpha(13) proteins.

Gene ID: 23365

NCBI Accession: NP_056128

UniProt: Q9NZN5

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling