DDX3X antibody (AA 550-600)

Catalog No. ABIN7452202

This Rabbit Polyclonal antibody specifically detects DDX3X in WB and IP. It exhibits reactivity toward Human.

Quick Overview for DDX3X antibody (AA 550-600) (ABIN7452202)

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX3X antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-DDX3X Antibody

Binding Specificity: AA 550-600

Purpose: Rabbit anti-DDX3 Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 550 and 600

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: 1:5,000 - 1:15,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for DDX3X

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Alternative Name: DDX3

Background: Background: DDX3 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX3 exhibits a variety of functions. DDX3 is a candidate tumor suppressor that suppresses tumor growth and transcriptionally activates the p21 promoter. DDX3 has been shown to play a role in translation as an eIF4E inhibitory protein that regulates translation initiation. These findings, as well as the observation that DDX3 is regulated by hepatitis C virus and is down-regulated in hepatocellular carcinoma, suggest an important role for DDX3 in growth-regulatory functions.

Gene ID: 1654

NCBI Accession: NP_001347

UniProt: O00571

Pathways: Ribonucleoprotein Complex Subunit Organization, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Ribosome Assembly