RNF138 antibody (AA 195-245)

Catalog No. ABIN7452246

This anti-RNF138 antibody is a Rabbit Polyclonal antibody detecting RNF138 in IP. Suitable for Human.

Quick Overview for RNF138 antibody (AA 195-245) (ABIN7452246)

Target: RNF138 (Ring Finger Protein 138 (RNF138))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF138 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-RNF138 Antibody

Binding Specificity: AA 195-245

Purpose: Rabbit anti-RNF138 Antibody, Affinity Purified

Predicted Reactivity: Bovine

Purification: Affinity Purified

Immunogen: between AA 195 and 245

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7452246 has not consistently performed satisfactorily when used for WB of RNF138 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RNF138.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RNF138

Target: RNF138 (Ring Finger Protein 138 (RNF138))

Alternative Name: RNF138

Background: Background: RING-finger protein 138 (RNF138) is an E3 ubiquitin ligase. Together with NLK, RNF138 is involved in the ubiquitination and degradation of TCF/LEF. RNF138 also exhibits auto-ubiquitination activity in combination with UBE2K. RNF138 may act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway [taken from the Universal Protein Resource (UniProt) Q8WVD3].

Gene ID: 51444

NCBI Accession: NP_057355

UniProt: Q8WVD3