RNF169 antibody (AA 200-250)

Catalog No. ABIN7452249

This anti-RNF169 antibody is a Rabbit Polyclonal antibody detecting RNF169 in IP. Suitable for Human.

Quick Overview for RNF169 antibody (AA 200-250) (ABIN7452249)

Target: RNF169 (Ring Finger Protein 169 (RNF169))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF169 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-RNF169 Antibody

Binding Specificity: AA 200-250

Purpose: Rabbit anti-RNF169 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 200 and 250

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7452249 has not performed satisfactorily when used for WB of RNF169 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RNF169.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RNF169

Target: RNF169 (Ring Finger Protein 169 (RNF169))

Alternative Name: RNF169

Background: Background: RING finger protein 169 (RNF169) is a probable E3 ubiquitin-protein ligase that acts as a negative regulator of the repair of double-strand breaks (DSBs) following DNA damage. RNF169 is recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSB repair [taken from the Universal Protein Resource (UniProt) Q8NCN4].

Gene ID: 254225

NCBI Accession: NP_001092108

UniProt: Q8NCN4