AFF4 antibody (AA 50-100)

Catalog No. ABIN7452779

The Rabbit Polyclonal anti-AFF4 antibody has been validated for IP. It is suitable to detect AFF4 in samples from Human.

Quick Overview for AFF4 antibody (AA 50-100) (ABIN7452779)

Target: AFF4 (AF4/FMR2 Family, Member 4 (AFF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFF4 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-AFF4 Antibody

Binding Specificity: AA 50-100

Purpose: Rabbit anti-MCEF Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 50 and 100

Isotype: IgG

Application Details

Application Notes:

IP: 5 - 15 μg/mg lysate

WB: Not recommended. ABIN7452779 has not performed satisfactorily when used for WB of MCEF in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MCEF.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for AFF4

Target: AFF4 (AF4/FMR2 Family, Member 4 (AFF4))

Alternative Name: MCEF

Background: Background: Major CDK9 elongation factor-associated protein (MCEF) is also known as AFF4 AF4/FMR2 family member 4. This family of proteins includes the AF4 proto-oncogene and FMR2 (fragile X E mental retardation syndrome) nuclear proteins. MCEF is a component of the cyclin-dependent kinase pair (CDK9/cyclin T1) complex. A chromosomal aberration involving AFF4 is found in acute lymphoblastic leukemia (ALL).

Gene ID: 27125

NCBI Accession: NP_055238

UniProt: Q9UHB7