RNA Polymerase II antibody (AA 754-804)

Catalog No. ABIN7453315

This Rabbit Polyclonal antibody specifically detects RNA Polymerase II in IP and ChIP. It exhibits reactivity toward Human.

Quick Overview for RNA Polymerase II antibody (AA 754-804) (ABIN7453315)

Target: RNA Polymerase II

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNA Polymerase II antibody is un-conjugated

Application: Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Product Details anti-RNA Polymerase II Antibody

Binding Specificity: AA 754-804

Purpose: Rabbit anti-RNA Polymerase II Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: Between AA 754 and 804

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7453315 has not performed satisfactorily when used for WB of RNA Polymerase II in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RNA Polymerase II.

ChIP-PCR: 8 μg per IP

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RNA Polymerase II

Target: RNA Polymerase II

Background: Background: RNA polymerase II is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II subunit B1 (RPB1) is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit [taken from NCBI Entrez Gene (Gene ID: 5430)].

Gene ID: 5430

NCBI Accession: NP_000928

UniProt: P24928