USP1 antibody (AA 450-500)

Catalog No. ABIN7453545

This anti-USP1 antibody is a Rabbit Polyclonal antibody detecting USP1 in IP and IHC (fp). Suitable for Human.

Quick Overview for USP1 antibody (AA 450-500) (ABIN7453545)

Target: USP1 (Ubiquitin Specific Peptidase 1 (USP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP1 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-USP1 Antibody

Binding Specificity: AA 450-500

Purpose: Rabbit anti-USP1 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 450 and 500

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7453545 has not performed satisfactorily when used for WB of USP1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of USP1.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for USP1

Target: USP1 (Ubiquitin Specific Peptidase 1 (USP1))

Alternative Name: USP1

Background: Background: Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 1 (USP1) has been found to play a role in the Fanconi anemia DNA repair pathway. USP1 specifically removes monoubiquitination from the Fanconi anemia D2 (FANCD2) protein and from the proliferating cell nuclear antigen (PCNA). Deubiquitination of FANCD2 and PCNA is critical to their role in the DNA damage response and DNA crosslink repair.

Gene ID: 7398

NCBI Accession: NP_003359

UniProt: O94782