FHL2 antibody (C-Term)

Catalog No. ABIN7453598

The Rabbit Polyclonal anti-FHL2 antibody is suitable to detect FHL2 in samples from Human and Mouse. It has been validated for WB, IP and IHC (fp).

Quick Overview for FHL2 antibody (C-Term) (ABIN7453598)

Target: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-FHL2 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-FHL2 Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 225 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 to 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for FHL2

Target: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Alternative Name: FHL2

Background: Background: FHL2 is a member of the four-and-a-half-LIM-domain protein family which consists of FHL1, FHL2, FHL3, FHL4, and ACT. The LIM domain is a cysteine-rich motif that mediates protein-protein interactions. FHL2 has been shown to function as a transcriptional cofactor and through its LIM domains, interact with a variety of factors which include the androgen receptor, CREB, AP-1, Runx3, SRF, FOXO1, BRCA1 and the HPV-16 E7 oncoprotein. FHL2 has been demonstrated to play an important role in heart and bone development and may influence cancer cell growth.

Gene ID: 2274

NCBI Accession: NP_963849

UniProt: Q14192

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha