DACH1 antibody (AA 740-760)

Catalog No. ABIN7453627

This Rabbit Polyclonal antibody specifically detects DACH1 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for DACH1 antibody (AA 740-760) (ABIN7453627)

Target: DACH1 (Dachshund 1 (DACH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DACH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-DACH1 Antibody

Binding Specificity: AA 740-760

Purpose: Rabbit anti-DACH1 Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 740 and 760

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for DACH1

Target: DACH1 (Dachshund 1 (DACH1))

Alternative Name: DACH1

Background: Background: Dachshund homolog 1 (Dach1) is a transcription factor that is involved in regulation of organogenesis. Dach1 seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Dach1 seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (by similarity) [taken from the Universal Protein Resource (UniProt) Q9UI36].

Gene ID: 1602

UniProt: Q9UI36

Pathways: Feeding Behaviour