BAG3 antibody (AA 300-350)

Catalog No. ABIN7453661

The Rabbit Polyclonal anti-BAG3 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect BAG3 in samples from Human.

Quick Overview for BAG3 antibody (AA 300-350) (ABIN7453661)

Target: BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-BAG3 Antibody

Binding Specificity: AA 300-350

Purpose: Rabbit anti-BAG3 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 300 and 350

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BAG3

Target: BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Alternative Name: BAG3

Background: Background: BAG3 (BCL2-associated athanogene 3) BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. BAG3 contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All three proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner [taken from NCBI Entrez Gene (Gene ID: 9531)].

Gene ID: 9531

NCBI Accession: NP_004272

UniProt: O95817