TDP2 antibody (AA 50-100)

Catalog No. ABIN7453801

This Rabbit Polyclonal antibody specifically detects TDP2 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for TDP2 antibody (AA 50-100) (ABIN7453801)

Target: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-TDP2 Antibody

Binding Specificity: AA 50-100

Purpose: Rabbit anti-TDP2 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 50 and 100

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for TDP2

Target: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Alternative Name: TDP2

Background: Background: TDP2 (5'-Tyr-DNA phosphodiesterase 2) is also known as TTRAP (TRAF and TNF receptor-associated protein). TTRAP is a DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. It catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. TTRAP also hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. TTRAP may also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation [taken from the Universal Protein Resource (UniProt) O95551].

Gene ID: 51567

NCBI Accession: NP_057698

UniProt: O95551