Polybromo 1 antibody (AA 1639-1689)

Catalog No. ABIN7453903

The Rabbit Polyclonal anti-Polybromo 1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect Polybromo 1 in samples from Human and Mouse.

Quick Overview for Polybromo 1 antibody (AA 1639-1689) (ABIN7453903)

Target: Polybromo 1 (PBRM1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Polybromo 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Polybromo 1 Antibody

Binding Specificity: AA 1639-1689

Purpose: Rabbit anti-PBRM1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 1639 and 1689

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Polybromo 1

Target: Polybromo 1 (PBRM1)

Alternative Name: PBRM1

Background: Background: The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PBRM1 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PBRM1 has been proposed to function as a tumor suppressor protein. Recently, PBRM1 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21.

Gene ID: 55193

UniProt: Q86U86