SMG9 antibody (AA 75-125)

Catalog No. ABIN7453938

Product Details anti-SMG9 Antibody

Target: SMG9 (Smg-9 Homolog, Nonsense Mediated mRNA Decay Factor (SMG9))

Binding Specificity: AA 75-125

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMG9 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-SMG9 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 75 and 125

Isotype: IgG

Application Details

Application Notes:

IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 5 - 10 μg/mg lysate

WB: Not recommended. ABIN7453938 has not performed satisfactorily when used for WB of SMG9 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of SMG9.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SMG9

Target: SMG9 (Smg-9 Homolog, Nonsense Mediated mRNA Decay Factor (SMG9))

Alternative Name: SMG9 (SMG9 Products)

Background: Background: SMG9 is a subunit of the SMG-1 complex. SMG-1 and Upf1 are part of a surveillance complex that participates in nonsense-mediated mRNA decay (NMD) to degrade mRNAs containing premature translation initiation codons (PTCs).

Gene ID: 56006

NCBI Accession: NP_061981

UniProt: Q9H0W8