APC antibody (C-Term)

Catalog No. ABIN7454385

This Rabbit Polyclonal antibody specifically detects APC in IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for APC antibody (C-Term) (ABIN7454385)

Target: APC (Adenomatous Polyposis Coli (APC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APC antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-APC Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-APC Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 2793 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended for crude lysate. Use at 1 μg/mL to blot enriched sources (e.g. Immunoprecipitates) of APC.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: APC

Background: Background: Adenomatosis polyposis coli (APC) is a tumor suppressor gene found to be mutated in most colon cancers and is the cause of familial adenomatous polyposis (FAP), an autosomal dominant disease that results in a predisposition to malignant colon cancer. APC has been shown to be involved in cell differentiation, migration, proliferation and adhesion. One function of APC involves its role as a scaffolding protein that regulates the phosphorylation and degradation of beta-catenin, a transcriptional regulator in the WNT signaling pathway. Additionally, APC has been found to be important in the regulation of microtubule arrays and mitotic spindle formation.

Gene ID: 324

NCBI Accession: NP_000029

UniProt: P25054

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization