DGCR8 antibody (AA 400-450)

Catalog No. ABIN7454450

This anti-DGCR8 antibody is a Rabbit Polyclonal antibody detecting DGCR8 in IP and IHC (fp). Suitable for Human.

Quick Overview for DGCR8 antibody (AA 400-450) (ABIN7454450)

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGCR8 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-DGCR8 Antibody

Binding Specificity: AA 400-450

Purpose: Rabbit anti-DGCR8 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 400 and 450

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-DGCR8 antibody A302-468A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for DGCR8

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Alternative Name: DGCR8

Background: Background: DiGeorge syndrome critical region 8 (DGCR8) is a component of the microprocessor complex that acts as a RNA- and heme-binding protein and is involved in the initial step of microRNA (miRNA) biogenesis. DGCR8 functions as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junctions. It directs RNASEN/DROSHA to cleave 11 bp away from the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs [taken from the Universal Protein Resource (UniProt) Q8WYQ5].

Gene ID: 54487

NCBI Accession: NP_073557

UniProt: Q8WYQ5

Pathways: Regulatory RNA Pathways