SUPT5H antibody (C-Term)

Catalog No. ABIN7454524

The Rabbit Polyclonal anti-SUPT5H antibody has been validated for WB and IHC (fp). It is suitable to detect SUPT5H in samples from Human.

Quick Overview for SUPT5H antibody (C-Term) (ABIN7454524)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT5H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-SUPT5H Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-SUPT5H Antibody, Affinity Purified

Predicted Reactivity: Mouse,Chicken,Orangutan

Purification: Affinity Purified

Immunogen: between AA 1040 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended. Use rabbit anti-SUPT5H antibody A300-868A.

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SUPT5H

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Alternative Name: SUPT5H

Background: Background: Transcription elongation is one step in the multistage process of transcription that involves the polymerization of nucleoside monophosphates into a ribonucleotide transcript catalyzed by the multisubunit complex RNA Pol II. Transcriptional regulation involves positive factors that promote sufficient elongation rates and processivity as well as negative elongation factors that repress transcription elongation. SUPT5H is a subunit of the DRB sensitivity inducing factor (DSIF) along with SUPT4H that functions as a negative elongation factor. DSIF associates with RNA Pol II and may inhibit elongation by pausing and terminating RNA Pol II activity.

Gene ID: 6829

NCBI Accession: NP_003160

UniProt: O00267