RNA Polymerase II antibody (pSer5)

Catalog No. ABIN7455032

This anti-RNA Polymerase II antibody is a Rabbit Polyclonal antibody detecting RNA Polymerase II in WB, IHC (fp) and IP. Suitable for Human and Mouse.

Quick Overview for RNA Polymerase II antibody (pSer5) (ABIN7455032)

Target: RNA Polymerase II

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNA Polymerase II antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Product Details anti-RNA Polymerase II Antibody

Binding Specificity: pSer5

Purpose: Rabbit anti-RNA Polymerase II, Phospho (S5) Antibody, Affinity Purified

Predicted Reactivity: D. melanogaster

Purification: Affinity Purified

Immunogen: surrounding serine 5

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RNA Polymerase II

Target: RNA Polymerase II

Background: Background: RNA polymerase II is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II subunit B1 (RPB1) is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit [taken from NCBI Entrez Gene (Gene ID: 5430)].

Gene ID: 5430

NCBI Accession: NP_000928

UniProt: P24928